Journal: Molecular Pain

Article Title: Systemic curcumin administration mitigates neuropathic pain in a rat model of trigeminal neuralgia by suppressing TNF-α and pSTAT3 signaling

doi: 10.1177/17448069261445281

Figure Lengend Snippet: GFAP expression across all groups. (a) The immunoreactivity of GFAP in IONC + curcumin group is decreased in the rat trigeminal ganglia after injection of curcumin (GFAP: green, DAPI: blue). (b) The intensity of GFAP is measured using corrected total cell fluorescence and showed a significantly lower intensity in IONC + curcumin group compared to IONC and IONC + vehicle groups. (c) mRNA expression of GFAP decreases after curcumin administration. Data are presented as mean ± SEM. Immunofluorescence staining, n = 3. qRT-PCR, n = 6. ** p < 0.005. Scale bar 50 µm.

Article Snippet: This was followed by overnight incubation at 4°C with anti-GFAP polyclonal antibody (1:500; Bioss Antibody, Boston, MA, USA), anti-ATF3 monoclonal antibody (1:500; Santa Cruz, CA, USA) and anti-phospho-STAT3 monoclonal antibody (1:100; Cell Signaling Technology, Denver, MA, USA).

Techniques: Expressing, Injection, Fluorescence, Immunofluorescence, Staining, Quantitative RT-PCR

Journal: Journal of biochemical and molecular toxicology

Article Title: Therapeutic effect of thymoquinone on brain damage caused by nonylphenol exposure in rats.

doi: 10.1002/jbt.23471

Figure Lengend Snippet: FIGURE 1 Graphical abstract. Cas‐3, caspase‐3; ELISA, enzyme‐linked immunosorbent assay; GFAP, glial fibrillary acidic protein; H&E, hematoxylin‐eosin; NeuN, neuronal nuclei; NGF, nerve growth factor; NOR, new object recognition; NP, nonylphenol; TQ, thymoquinone.

Article Snippet: Cas‐3 (Cat. No: E‐AB‐13815; Elabscience), NGF (Cat. No: E‐AB‐32239; Elabscience), GFAP (Cat. No: NB120‐16997; Novus Biologicals), and NeuN (Cat. No: FNab05669; Finetest) antibodies were prepared and instilled into the tissues.

Techniques: Enzyme-linked Immunosorbent Assay

Journal: Journal of biochemical and molecular toxicology

Article Title: Therapeutic effect of thymoquinone on brain damage caused by nonylphenol exposure in rats.

doi: 10.1002/jbt.23471

Figure Lengend Snippet: FIGURE 4 Immunohistochemical staining of NeuN, GFAP, Cas‐3, and NGF in experimental groups. Black arrow indicates immunohistochemically stained structures. The yellow arrow indicate structures that are not or understained immunohistochemically. (A) Control, (B) nonylphenol, (C) nonylphenol + thymoquinone, (D) thymoquinone. Immunohistochemical staining, Olympus microscope, ×400. Cas‐ 3, caspase‐3; GFAP, glial fibrillary acidic protein; NeuN, neuronal nuclei; NGF, nerve growth factor.

Article Snippet: Cas‐3 (Cat. No: E‐AB‐13815; Elabscience), NGF (Cat. No: E‐AB‐32239; Elabscience), GFAP (Cat. No: NB120‐16997; Novus Biologicals), and NeuN (Cat. No: FNab05669; Finetest) antibodies were prepared and instilled into the tissues.

Techniques: Immunohistochemical staining, Staining, Control, Microscopy

Journal: Journal of biochemical and molecular toxicology

Article Title: Therapeutic effect of thymoquinone on brain damage caused by nonylphenol exposure in rats.

doi: 10.1002/jbt.23471

Figure Lengend Snippet: FIGURE 5 Statistical analysis of NeuN, GFAP, Cas‐3, and NGF immunoreactivity of the experimental groups. Cas‐3, caspase‐3; GFAP, glial fibrillary acidic protein; NeuN, neuronal nuclei; NGF, nerve growth factor; NP, nonylphenol; TQ, thymoquinone.

Article Snippet: Cas‐3 (Cat. No: E‐AB‐13815; Elabscience), NGF (Cat. No: E‐AB‐32239; Elabscience), GFAP (Cat. No: NB120‐16997; Novus Biologicals), and NeuN (Cat. No: FNab05669; Finetest) antibodies were prepared and instilled into the tissues.

Techniques:

Journal: Nature Communications

Article Title: Neuropeptide SP protects against colitis and linked anxiety-like behavior through the putative roles of gut microbiota and metabolite inositol

doi: 10.1038/s41467-025-67904-0

Figure Lengend Snippet: a Diagram illustrating the mouse model of colitis employed in this study, and inositol treatments are indicated (Some schematic elements were created by Figdraw.com). b Daily body weight changes. n = 8 mice/group. c Diseases activity index. n = 6 mice/group. d Macroscopic pictures of colons. e H&E-stained colon sections. Scale bar = 100 μm. n = 5 independent experiments. f Immunofluorescence staining for E-cadherin (red)/DAPI (blue) in colon tissues. Scale bar = 100 μm. n = 4 independent experiments. g The mRNA level of inflammatory cytokines ( IL-1β , IL-6 and TNF-α ) in the colon. n = 4 mice/group. h , i Representative movement tracks in the open field test and related bar graphs (Distance traveled, distance of center region and speed). n = 6 mice/group. j , k Track plot of the elevated plus maze, and statistical analysis including percentage of time spent in the open arms and percentage of times entering the open arms. n = 6 mice/group. l Representative H&E-stained hippocampus sections of three groups. Scale bar = 100 μm. n = 6 independent experiments. m Photomicrographs of Nissl staining in the hippocampus. Scale bar = 100 μm. n = 6 independent experiments. n The mRNA expressions of downstream cytokines ( IL-6 , IL-1β , TNF-α and IL-10 ) in the hippocampus tissue. n = 5 mice/group. o Representative immunohistochemistry images of Iba-1 in hippocampus, Scale bar = 100 μm. n = 3 independent experiments. p The concentration of GABA in hippocampus tissue. n = 6 mice/group. q The mRNA expression of GABA A Rα1 in hippocampus. n = 5 mice/group. r The protein level of GABA B R detected by western blot. n = 3 independent experiments. s Double immunofluorescence staining for GFAP (red)/GAT1 (green) in the DG and CA1 regions of hippocampus tissue. Scale bar = 50 μm. n = 3 independent experiments. Data were presented as means ± SD. For body weight change, two-way repeated-measures ANOVA was performed and the rest of the statistics was analyzed using one-way ANOVA followed by Tukey’s multiple comparisons test. * P ≤ 0.05, ** P ≤ 0.01, *** P ≤ 0.001. Source data are provided as a file.

Article Snippet: Briefly, after deparaffinization, rehydration, and antigen retrieval, slides were blocked with donkey serum for 30 min at room temperature following incubation with primary antibodies against GFAP (1:1000, A8335, 1:200, NB100-53809, USA), Iba-1 (DF6442, 1:200, Affinity, China), E-cadherin (PB9561, 1:200, Boster, China), Mucin 2 (bsm-60016R, 1:100, Bioss, China), GABA A Rα1 (1:200, 12708-1-AP, Proteintech, China), calcium/calmodulin-dependent protein kinase II (CaMKII) (1:50, ab76703, Abcam, UK) and p-IKBα (1:100, WLH3930, Wanleibio, China) at 4 C overnight.

Techniques: Activity Assay, Staining, Immunofluorescence, Immunohistochemistry, Concentration Assay, Expressing, Western Blot, Double Immunofluorescence Staining

Journal: PLoS ONE

Article Title: Gradient Index Microlens Implanted in Prefrontal Cortex of Mouse Does Not Affect Behavioral Test Performance over Time

doi: 10.1371/journal.pone.0146533

Figure Lengend Snippet: Significance of factors in presurgical tests.

Article Snippet: Sections were incubated with the primary antibody, mouse anti-glial fibrillary acidic protein (GFAP) (clone N206A/8; 1:500 dilution in blocking solution; NIH Neuromab Facility, UC Davis, USA) overnight at RT.

Techniques:

Journal: PLoS ONE

Article Title: Gradient Index Microlens Implanted in Prefrontal Cortex of Mouse Does Not Affect Behavioral Test Performance over Time

doi: 10.1371/journal.pone.0146533

Figure Lengend Snippet: Glial cells in layer 5 of the prefrontal cortex of GFAP-GFP mice imaged using implanted ultrathin GRIN lenses and multiphoton microscopy. Digital zoom images were acquired to show details of cells; the full field of view was 180 μm. A. Glial cells expressing GFP under the GFAP promoter are wrapped around a blood vessel counterstained with dextran-Texas red dye. This image is a z-projection of 3 sequential image planes with a 1-μm step size. B. This image is a z-projection of 2 image planes, which was done to average out noise and to better visualize cellular processes. C. Single plane image of glial cells in a different mouse. Scale bars denote 5 μm.

Article Snippet: Sections were incubated with the primary antibody, mouse anti-glial fibrillary acidic protein (GFAP) (clone N206A/8; 1:500 dilution in blocking solution; NIH Neuromab Facility, UC Davis, USA) overnight at RT.

Techniques: Microscopy, Expressing

Journal: PLoS ONE

Article Title: Gradient Index Microlens Implanted in Prefrontal Cortex of Mouse Does Not Affect Behavioral Test Performance over Time

doi: 10.1371/journal.pone.0146533

Figure Lengend Snippet: A. Coronal slice containing the track of an implanted GRIN lens is outlined with a dotted yellow line. The implant depth was 935 μm below the pial surface. B. Coronal slice stained with anti-GFAP antibody. The white dashed lines denote the mean thickness of the glial scar under the lens, which was 45.9 ± 4.2 μm (mean ± SEM, 40 areas, 3 mice). The working distance of the GRIN lens, which was 125 μm, is much longer than the thickness of the scar. Therefore, the cells within 125 μm of the lens are too close for inclusion in any in vivo images. A solid light yellow line denotes the region that can be visualized with the GRIN lens and multiphoton microscopy (imaging region). The GFAP+ cells in the imaging region were relatively few and exhibited only weak immunoreactivity compared with the scar. The inset is an enlargement of this area (inset was brightened and contrast was increased to show relatively faint staining). C. An area in the ventrolateral cortex of the contralateral hemisphere was used as a negative control. A solid light yellow line denotes the region which is at the same depth and size as the imaging region and in the same orientation with respect to the pial surface. The inset is an enlargement of this area (inset was brightened and contrast was increased to show relatively faint staining). The number of GFAP+ cells were not significantly different for the imaged region and the negative control region (p = 0.36). Scale bars are 200 μm for A and 100 μm for B and C.

Article Snippet: Sections were incubated with the primary antibody, mouse anti-glial fibrillary acidic protein (GFAP) (clone N206A/8; 1:500 dilution in blocking solution; NIH Neuromab Facility, UC Davis, USA) overnight at RT.

Techniques: Staining, In Vivo, Microscopy, Imaging, Negative Control